pegfp c2 mammalian expression vector (TaKaRa)
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pegfp c2 mammalian expression vector
Pegfp C2 Mammalian Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mammalian+expression+vector+pegfp+c2/pmCherry+Vector/pmc03069065-117-32-36
Average 96 stars, based on 619 article reviews
Pegfp C2 Mammalian Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mammalian+expression+vector+pegfp+c2/pmCherry+Vector/pmc03069065-117-32-36
Average 96 stars, based on 619 article reviews
pegfp c2 mammalian expression vector - by Bioz Stars,
2026-10
96/100 stars
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Expressing:Article Title: Matrix protein 2 of influenza A virus blocks autophagosome fusion with lysosomes Article Snippet: Inhibitors, synthetic peptides and cytokines Chloroquine and amantidine were purchased from Sigma, St. Louis, MO. .. Expression plasmids and lentiviral constructs The cDNA of human MAP1LC3B sequence ( {"type":"entrez-nucleotide","attrs":{"text":"NM_022818","term_id":"197382745","term_text":"NM_022818"}} NM_022818 ) was PCR-amplified from a human B-LCL by RT-PCR with gene specific primers and cloned into the multiple cloning site of the Article Title: LIN28B is highly expressed in atypical teratoid/rhabdoid tumor (AT/RT) and suppressed through the restoration of SMARCB1 Article Snippet: The siRNA transfections were performed using Lipofectamine ® RNAiMAX (Life Technologies) according to the manufacturer’s instructions. .. To construct SMARCB1, full-length SMARCB1 was cloned into the Article Title: The Role of the PDZ Protein GIPC in Regulating NMDA Receptor Trafficking Article Snippet: .. GIPC full-length cDNA, GIPC-N PDZ (amino acids 1–225), GIPC-C PDZ (amino acids 125–333), GIPC-N (amino acids 1–124), GIPC-C (amino acids 226 –333), and GIPC-PDZ (amino acids 226 –333) were amplified from the mouse brain cDNA library (Clontech, Palo Alto, CA) using PCR and subcloned into the Article Title: Htra2-β 1 stimulates an exonic splicing enhancer and can restore full-length SMN expression to survival motor neuron 2 ( SMN2 ) Article Snippet: .. The cDNA of Htra2-β1 and Htra2-β3 ( 17 ), Htra2-α ( 18 ), SF2/ASF ( 19 ), SRp30c ( 20 ), U2AF65 ( 21 ), SAF-B ( 22 ), PTB ( 23 ), SLM-2 ( 24 ), SF1(25), CLK2 and CLK2-KR ( 26 ), and YT521B ( 27 ) were subcloned into the Article Title: The Role of the PDZ Protein GIPC in Regulating NMDA Receptor Trafficking Article Snippet: .. GIPC full-length cDNA, GIPC-N+PDZ (amino acids 1–225), GIPC-C+PDZ (amino acids 125–333), GIPC-N (amino acids 1–124), GIPC-C (amino acids 226–333), and GIPC-PDZ (amino acids 226–333) were amplified from the mouse brain cDNA library (Clontech, Palo Alto, CA) using PCR and subcloned into the Article Title: Distinct roles of parafibromin in the extracellular environment, cytoplasm and nucleus of osteosarcoma cells Article Snippet: Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Hyclone, Logan, UT, USA) supplemented with 10% heat-inactivated fetal calf serum (FCS), 2 mM glutamine, penicillin (100 U/ml), and streptomycin (100 mg/ml) at 37°C with 5% CO 2 . .. Parafibromin expression vector was constructed by PCR amplification of human CDC73 natural ORF mammalian expression plasmid (HG11656-UT, Sino Biological Inc, Beijing, China) utilizing the primers (Sangon Biotech, Shanghai, China): 5’-TTCGAATTCATGGCGGACGTGCTTAGCGTCC-3’ (forward) and 5’-TACCGTCGACATAATTCAGAATCTCAAGTGC-3’ (reverse), and cloning into the Article Title: Lysosomal chymotrypsin induces mitochondrial fission in apoptotic cells by proteolytic activation of calcineurin Article Snippet: .. The cDNAs for full-length calcineurin and truncated calcineurin (1-410 lacking the C-terminal autoinhibitory domain) were subcloned into the Article Title: Identification of Glypican-3 as a potential metastasis suppressor gene in gastric cancer Article Snippet: .. To overexpress GPC3, the coding sequence of human transcript variant 1 was amplified from a cDNA library with Pyrobest DNA Polymerase (Takara, Japan) and cloned into the Construct:Article Title: Matrix protein 2 of influenza A virus blocks autophagosome fusion with lysosomes Article Snippet: Inhibitors, synthetic peptides and cytokines Chloroquine and amantidine were purchased from Sigma, St. Louis, MO. .. Expression plasmids and lentiviral constructs The cDNA of human MAP1LC3B sequence ( {"type":"entrez-nucleotide","attrs":{"text":"NM_022818","term_id":"197382745","term_text":"NM_022818"}} NM_022818 ) was PCR-amplified from a human B-LCL by RT-PCR with gene specific primers and cloned into the multiple cloning site of the Article Title: LIN28B is highly expressed in atypical teratoid/rhabdoid tumor (AT/RT) and suppressed through the restoration of SMARCB1 Article Snippet: The siRNA transfections were performed using Lipofectamine ® RNAiMAX (Life Technologies) according to the manufacturer’s instructions. .. To construct SMARCB1, full-length SMARCB1 was cloned into the Article Title: Distinct roles of parafibromin in the extracellular environment, cytoplasm and nucleus of osteosarcoma cells Article Snippet: Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Hyclone, Logan, UT, USA) supplemented with 10% heat-inactivated fetal calf serum (FCS), 2 mM glutamine, penicillin (100 U/ml), and streptomycin (100 mg/ml) at 37°C with 5% CO 2 . .. Parafibromin expression vector was constructed by PCR amplification of human CDC73 natural ORF mammalian expression plasmid (HG11656-UT, Sino Biological Inc, Beijing, China) utilizing the primers (Sangon Biotech, Shanghai, China): 5’-TTCGAATTCATGGCGGACGTGCTTAGCGTCC-3’ (forward) and 5’-TACCGTCGACATAATTCAGAATCTCAAGTGC-3’ (reverse), and cloning into the Sequencing:Article Title: Matrix protein 2 of influenza A virus blocks autophagosome fusion with lysosomes Article Snippet: Inhibitors, synthetic peptides and cytokines Chloroquine and amantidine were purchased from Sigma, St. Louis, MO. .. Expression plasmids and lentiviral constructs The cDNA of human MAP1LC3B sequence ( {"type":"entrez-nucleotide","attrs":{"text":"NM_022818","term_id":"197382745","term_text":"NM_022818"}} NM_022818 ) was PCR-amplified from a human B-LCL by RT-PCR with gene specific primers and cloned into the multiple cloning site of the Article Title: Identification of Glypican-3 as a potential metastasis suppressor gene in gastric cancer Article Snippet: .. To overexpress GPC3, the coding sequence of human transcript variant 1 was amplified from a cDNA library with Pyrobest DNA Polymerase (Takara, Japan) and cloned into the Polymerase Chain Reaction:Article Title: Matrix protein 2 of influenza A virus blocks autophagosome fusion with lysosomes Article Snippet: Inhibitors, synthetic peptides and cytokines Chloroquine and amantidine were purchased from Sigma, St. Louis, MO. .. Expression plasmids and lentiviral constructs The cDNA of human MAP1LC3B sequence ( {"type":"entrez-nucleotide","attrs":{"text":"NM_022818","term_id":"197382745","term_text":"NM_022818"}} NM_022818 ) was PCR-amplified from a human B-LCL by RT-PCR with gene specific primers and cloned into the multiple cloning site of the Article Title: The Role of the PDZ Protein GIPC in Regulating NMDA Receptor Trafficking Article Snippet: .. GIPC full-length cDNA, GIPC-N PDZ (amino acids 1–225), GIPC-C PDZ (amino acids 125–333), GIPC-N (amino acids 1–124), GIPC-C (amino acids 226 –333), and GIPC-PDZ (amino acids 226 –333) were amplified from the mouse brain cDNA library (Clontech, Palo Alto, CA) using PCR and subcloned into the Article Title: The Role of the PDZ Protein GIPC in Regulating NMDA Receptor Trafficking Article Snippet: .. GIPC full-length cDNA, GIPC-N+PDZ (amino acids 1–225), GIPC-C+PDZ (amino acids 125–333), GIPC-N (amino acids 1–124), GIPC-C (amino acids 226–333), and GIPC-PDZ (amino acids 226–333) were amplified from the mouse brain cDNA library (Clontech, Palo Alto, CA) using PCR and subcloned into the Article Title: Distinct roles of parafibromin in the extracellular environment, cytoplasm and nucleus of osteosarcoma cells Article Snippet: Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Hyclone, Logan, UT, USA) supplemented with 10% heat-inactivated fetal calf serum (FCS), 2 mM glutamine, penicillin (100 U/ml), and streptomycin (100 mg/ml) at 37°C with 5% CO 2 . .. Parafibromin expression vector was constructed by PCR amplification of human CDC73 natural ORF mammalian expression plasmid (HG11656-UT, Sino Biological Inc, Beijing, China) utilizing the primers (Sangon Biotech, Shanghai, China): 5’-TTCGAATTCATGGCGGACGTGCTTAGCGTCC-3’ (forward) and 5’-TACCGTCGACATAATTCAGAATCTCAAGTGC-3’ (reverse), and cloning into the Reverse Transcription Polymerase Chain Reaction:Article Title: Matrix protein 2 of influenza A virus blocks autophagosome fusion with lysosomes Article Snippet: Inhibitors, synthetic peptides and cytokines Chloroquine and amantidine were purchased from Sigma, St. Louis, MO. .. Expression plasmids and lentiviral constructs The cDNA of human MAP1LC3B sequence ( {"type":"entrez-nucleotide","attrs":{"text":"NM_022818","term_id":"197382745","term_text":"NM_022818"}} NM_022818 ) was PCR-amplified from a human B-LCL by RT-PCR with gene specific primers and cloned into the multiple cloning site of the Clone Assay:Article Title: Matrix protein 2 of influenza A virus blocks autophagosome fusion with lysosomes Article Snippet: Inhibitors, synthetic peptides and cytokines Chloroquine and amantidine were purchased from Sigma, St. Louis, MO. .. Expression plasmids and lentiviral constructs The cDNA of human MAP1LC3B sequence ( {"type":"entrez-nucleotide","attrs":{"text":"NM_022818","term_id":"197382745","term_text":"NM_022818"}} NM_022818 ) was PCR-amplified from a human B-LCL by RT-PCR with gene specific primers and cloned into the multiple cloning site of the Article Title: LIN28B is highly expressed in atypical teratoid/rhabdoid tumor (AT/RT) and suppressed through the restoration of SMARCB1 Article Snippet: The siRNA transfections were performed using Lipofectamine ® RNAiMAX (Life Technologies) according to the manufacturer’s instructions. .. To construct SMARCB1, full-length SMARCB1 was cloned into the Article Title: Identification of Glypican-3 as a potential metastasis suppressor gene in gastric cancer Article Snippet: .. To overexpress GPC3, the coding sequence of human transcript variant 1 was amplified from a cDNA library with Pyrobest DNA Polymerase (Takara, Japan) and cloned into the Cloning:Article Title: Matrix protein 2 of influenza A virus blocks autophagosome fusion with lysosomes Article Snippet: Inhibitors, synthetic peptides and cytokines Chloroquine and amantidine were purchased from Sigma, St. Louis, MO. .. Expression plasmids and lentiviral constructs The cDNA of human MAP1LC3B sequence ( {"type":"entrez-nucleotide","attrs":{"text":"NM_022818","term_id":"197382745","term_text":"NM_022818"}} NM_022818 ) was PCR-amplified from a human B-LCL by RT-PCR with gene specific primers and cloned into the multiple cloning site of the Article Title: Distinct roles of parafibromin in the extracellular environment, cytoplasm and nucleus of osteosarcoma cells Article Snippet: Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Hyclone, Logan, UT, USA) supplemented with 10% heat-inactivated fetal calf serum (FCS), 2 mM glutamine, penicillin (100 U/ml), and streptomycin (100 mg/ml) at 37°C with 5% CO 2 . .. Parafibromin expression vector was constructed by PCR amplification of human CDC73 natural ORF mammalian expression plasmid (HG11656-UT, Sino Biological Inc, Beijing, China) utilizing the primers (Sangon Biotech, Shanghai, China): 5’-TTCGAATTCATGGCGGACGTGCTTAGCGTCC-3’ (forward) and 5’-TACCGTCGACATAATTCAGAATCTCAAGTGC-3’ (reverse), and cloning into the Plasmid Preparation:Article Title: Matrix protein 2 of influenza A virus blocks autophagosome fusion with lysosomes Article Snippet: Inhibitors, synthetic peptides and cytokines Chloroquine and amantidine were purchased from Sigma, St. Louis, MO. .. Expression plasmids and lentiviral constructs The cDNA of human MAP1LC3B sequence ( {"type":"entrez-nucleotide","attrs":{"text":"NM_022818","term_id":"197382745","term_text":"NM_022818"}} NM_022818 ) was PCR-amplified from a human B-LCL by RT-PCR with gene specific primers and cloned into the multiple cloning site of the Article Title: The Role of the PDZ Protein GIPC in Regulating NMDA Receptor Trafficking Article Snippet: .. GIPC full-length cDNA, GIPC-N PDZ (amino acids 1–225), GIPC-C PDZ (amino acids 125–333), GIPC-N (amino acids 1–124), GIPC-C (amino acids 226 –333), and GIPC-PDZ (amino acids 226 –333) were amplified from the mouse brain cDNA library (Clontech, Palo Alto, CA) using PCR and subcloned into the Article Title: Htra2-β 1 stimulates an exonic splicing enhancer and can restore full-length SMN expression to survival motor neuron 2 ( SMN2 ) Article Snippet: .. The cDNA of Htra2-β1 and Htra2-β3 ( 17 ), Htra2-α ( 18 ), SF2/ASF ( 19 ), SRp30c ( 20 ), U2AF65 ( 21 ), SAF-B ( 22 ), PTB ( 23 ), SLM-2 ( 24 ), SF1(25), CLK2 and CLK2-KR ( 26 ), and YT521B ( 27 ) were subcloned into the Article Title: The Role of the PDZ Protein GIPC in Regulating NMDA Receptor Trafficking Article Snippet: .. GIPC full-length cDNA, GIPC-N+PDZ (amino acids 1–225), GIPC-C+PDZ (amino acids 125–333), GIPC-N (amino acids 1–124), GIPC-C (amino acids 226–333), and GIPC-PDZ (amino acids 226–333) were amplified from the mouse brain cDNA library (Clontech, Palo Alto, CA) using PCR and subcloned into the Article Title: Distinct roles of parafibromin in the extracellular environment, cytoplasm and nucleus of osteosarcoma cells Article Snippet: Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Hyclone, Logan, UT, USA) supplemented with 10% heat-inactivated fetal calf serum (FCS), 2 mM glutamine, penicillin (100 U/ml), and streptomycin (100 mg/ml) at 37°C with 5% CO 2 . .. Parafibromin expression vector was constructed by PCR amplification of human CDC73 natural ORF mammalian expression plasmid (HG11656-UT, Sino Biological Inc, Beijing, China) utilizing the primers (Sangon Biotech, Shanghai, China): 5’-TTCGAATTCATGGCGGACGTGCTTAGCGTCC-3’ (forward) and 5’-TACCGTCGACATAATTCAGAATCTCAAGTGC-3’ (reverse), and cloning into the Article Title: Identification of Glypican-3 as a potential metastasis suppressor gene in gastric cancer Article Snippet: .. To overexpress GPC3, the coding sequence of human transcript variant 1 was amplified from a cDNA library with Pyrobest DNA Polymerase (Takara, Japan) and cloned into the Amplification:Article Title: The Role of the PDZ Protein GIPC in Regulating NMDA Receptor Trafficking Article Snippet: .. GIPC full-length cDNA, GIPC-N PDZ (amino acids 1–225), GIPC-C PDZ (amino acids 125–333), GIPC-N (amino acids 1–124), GIPC-C (amino acids 226 –333), and GIPC-PDZ (amino acids 226 –333) were amplified from the mouse brain cDNA library (Clontech, Palo Alto, CA) using PCR and subcloned into the Article Title: The Role of the PDZ Protein GIPC in Regulating NMDA Receptor Trafficking Article Snippet: .. GIPC full-length cDNA, GIPC-N+PDZ (amino acids 1–225), GIPC-C+PDZ (amino acids 125–333), GIPC-N (amino acids 1–124), GIPC-C (amino acids 226–333), and GIPC-PDZ (amino acids 226–333) were amplified from the mouse brain cDNA library (Clontech, Palo Alto, CA) using PCR and subcloned into the Article Title: Distinct roles of parafibromin in the extracellular environment, cytoplasm and nucleus of osteosarcoma cells Article Snippet: Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Hyclone, Logan, UT, USA) supplemented with 10% heat-inactivated fetal calf serum (FCS), 2 mM glutamine, penicillin (100 U/ml), and streptomycin (100 mg/ml) at 37°C with 5% CO 2 . .. Parafibromin expression vector was constructed by PCR amplification of human CDC73 natural ORF mammalian expression plasmid (HG11656-UT, Sino Biological Inc, Beijing, China) utilizing the primers (Sangon Biotech, Shanghai, China): 5’-TTCGAATTCATGGCGGACGTGCTTAGCGTCC-3’ (forward) and 5’-TACCGTCGACATAATTCAGAATCTCAAGTGC-3’ (reverse), and cloning into the Article Title: Identification of Glypican-3 as a potential metastasis suppressor gene in gastric cancer Article Snippet: .. To overexpress GPC3, the coding sequence of human transcript variant 1 was amplified from a cDNA library with Pyrobest DNA Polymerase (Takara, Japan) and cloned into the cDNA Library Assay:Article Title: The Role of the PDZ Protein GIPC in Regulating NMDA Receptor Trafficking Article Snippet: .. GIPC full-length cDNA, GIPC-N PDZ (amino acids 1–225), GIPC-C PDZ (amino acids 125–333), GIPC-N (amino acids 1–124), GIPC-C (amino acids 226 –333), and GIPC-PDZ (amino acids 226 –333) were amplified from the mouse brain cDNA library (Clontech, Palo Alto, CA) using PCR and subcloned into the Article Title: The Role of the PDZ Protein GIPC in Regulating NMDA Receptor Trafficking Article Snippet: .. GIPC full-length cDNA, GIPC-N+PDZ (amino acids 1–225), GIPC-C+PDZ (amino acids 125–333), GIPC-N (amino acids 1–124), GIPC-C (amino acids 226–333), and GIPC-PDZ (amino acids 226–333) were amplified from the mouse brain cDNA library (Clontech, Palo Alto, CA) using PCR and subcloned into the Article Title: Identification of Glypican-3 as a potential metastasis suppressor gene in gastric cancer Article Snippet: .. To overexpress GPC3, the coding sequence of human transcript variant 1 was amplified from a cDNA library with Pyrobest DNA Polymerase (Takara, Japan) and cloned into the Variant Assay:Article Title: Identification of Glypican-3 as a potential metastasis suppressor gene in gastric cancer Article Snippet: .. To overexpress GPC3, the coding sequence of human transcript variant 1 was amplified from a cDNA library with Pyrobest DNA Polymerase (Takara, Japan) and cloned into the |
